大赢家彩票官网

设为首页  |   加入收藏  |   联系我们
产品目录
代理品牌
酶联免疫学类
分子学类
生化学类
ELISA试剂盒
进口ELISA试剂盒
人ELISA试剂盒
国产ELISA试剂盒
人ELISA试剂盒价格
小鼠ELISA试剂盒
大鼠ELISA试剂盒
豚鼠ELISA试剂盒
猪ELISA试剂盒
鸡ELISA试剂盒
兔ELISA试剂盒
Spectrum试剂
进口试剂
生化试剂
USP级试剂
Amresco试剂
氨基酸试剂
酶试剂
蛋白质试剂
抗生素试剂
植物激素及核酸试剂
其他生化试剂
缓冲剂试剂
表面活性剂
维生素试剂
色素试剂
碳水化合物试剂
澳洲血清
胎牛血清
美国进口血清
动物血清
人血清
ABCAM抗体
一抗
二抗
进口标准品
对照品
标准品
食品检测试剂盒
检测试剂盒
检测试纸条
PCR检测试剂盒
ELISA快速检测试剂盒
食品与农产品安全速测试剂盒
进口其他检测试剂盒
金标试剂盒
性病试剂盒
传染病试剂盒
优生优育试剂盒
自身免疫试剂盒
寄生虫试剂盒
微生物培养试剂盒
染色液试剂盒
免疫化学品
免疫球蛋白抗原
纯化抗体
荧光素标记抗体
酶标记抗体
酶标亲合素及生物素化抗体
免疫血清
其它方法测试盒
速率法测试盒
终点法测试盒
比色法测试盒
染色测试盒
其它测试盒
农药标准品
杀虫剂标准品
杀菌剂标准品
除草剂标准品
杀鼠剂标准品
Spectrum试剂新品上市
联系我们
  • 地址:上海市杨浦区控江路1029弄B幢105室
  • 电话:021-60531073,60520348
  • 传真:021-64881400
  • 手机:18221656311,13296034073,13296070348
  • 联系人:钱经理
  • 网址:www.njxjlmy.com
  • 邮箱:2885617636@qq.com
你当前的位置:产品详细展示

Chicken TNF-α ELISA Kit

型号:  所在地:美洲  发布时间:  参展人数:622

Chicken TNF-α ELISA Kit

 详细介绍

INTENDED USE AND TEST PRINCIPLE
This TNF-α ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of TNF-α in the sample, this TNF-α ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus TNF-α concentration. The concentration of TNF-α in the samples is then determined by comparing the O.D. of the samples to the standard curve.

SAMPLE COLLECTION AND STORAGES
Serum - Use a serum separator tube and allow samples to clot for 2 hours at room temperature or overnight at 4℃ before centrifugation for 20 minutes at approximately 2000×g. Remove serum and assay imtely or aliquot and store samples at -20℃. Avoid repeated freeze-thaw cycles
Plasma - Collect plasma using heparin as an anticoagulant. Centrifuge samples for 30 minutes at 2000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃. Avoid repeated freeze-thaw cycles.
Tissue homogenates - For general inion, hemolysis blood may affect the result, so you should rinse the tissues with ice-cold PBS (0.01M, pH=7.4) to remove excess blood thoroughly. Tissue pieces should be weighed and then minced to small pieces which will be homogenized in PBS (the volume depends on the weight of the tissue. 9mL PBS would be appropriate to 1 gram tissue pieces. Some protease inhibitor is recommended to add into the PBS.) with a glass homogenizer on ice. To further break the cells, you can sonicate the suspension with an ultrasonic cell disrupter or subject it to freeze-thaw cycles. The homogenates are then centrifugated for 5minutes at 5000×g to get the supernate.
Cell culture supernates, tissue homogenate and other biological fluids - Remove particulates by centrifugation and assay imtely or aliquot and store samples at -20℃. Avoid repeated freeze-thaw cycles.
Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.

CALCULATION OF RESULTS
1.This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (X) axis versus the corresponding concentration on the horizontal (Y) axis.
2.First, calculate the mean O.D. value for each standard and sample. All O.D. Values are subtracted by the mean value of the balnk well before result interpretation. Construct the standard curve using graph paper or statistical software.
3.To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.
4.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
5.Intra-assay CV(%) is less than 10% and Inter-assay CV(%) is less than 15%.
6.Assay range: 2.5 pg/mL – 80 pg/mL.
7. Sensitivity: The minimum detectable dose of Chicken TNF-α is typically less than 0.1 pg/mL.
8. Cross-reactivity: This assay recognizes recombinant and natural Chicken TNF-α. No significant cross-reactivity or interference was observed.
9. Storage: 2-8℃ (Use frequently); six months (-20℃)。
10. Standard curve


标签:Chicken   TNF-α   ELISA   Kit   
联系我们
上海恒远生物技术发展有限公司
钱经理(联系我时请说是在阿仪网)
电话:021-60531073,60520348    手机:18221656311,13296034073,13296070348
传真: 021-64881400
地址:上海市杨浦区控江路1029弄B幢105室
邮箱:通过本站在线发送邮件给展商
公司网站:www.njxjlmy.com
商铺网址:http://www.app17.com/C60514/
邮编:200093
产品询价
    留言框
    感兴趣的产品: *
    您的单位: *  
    您的姓名: *
    联系电话: *
    详细地址:
    常用邮箱:
    您的任何要求、意见或建议:
    *
    验证码: *
          

上海恒远生物技术发展有限公司主营:ELISA试剂盒,ELISA试剂盒价格,国产ELISA试剂盒,进口ELISA试剂盒

公司名称:上海恒远生物技术发展有限公司 公司地址:上海市杨浦区控江路1029弄B幢105室 邮政编码:200093 邮箱:2885617636@qq.com
联系人:钱经理 联系电话:021-60531073,60520348 ICP备案号:沪ICP备11004148号-11 技术支持:阿仪网总访问量:4783127
阿仪网推荐收藏该企业网站

沪公网安备 9号彩票www.njxjlmy.com版权所有

友情链接:现金网  现金网官网  CNC娱乐  CNC娱乐  现金网  现金网  现金网  CNC娱乐  现金网  现金网官网  

免责声明: 本站资料及图片来源互联网文章,本网不承担任何由内容信息所引起的争议和法律责任。所有作品版权归原创作者所有,与本站立场无关,如用户分享不慎侵犯了您的权益,请联系我们告知,我们将做删除处理!